Diabetes mellitus (DM) is a metabolic disorder due to altered carbohydrate, protein and fat metabolism. It is also well known as endocrine disorder because to insufficiency of the hormone, insulin which is secreted by the beta cells of pancreas. Diabetes is characterized by polyuria [frequent urination], polydipsia [increased thirst], polyphagia [increased hunger] and loss of weight, in later peripheral neuritis on palm and sole. Depending upon the etiology Diabetes is divided into 2 Types. That is 1.Insulin dependent and 2.Non-Insulin Dependent (IDDM and NIIDDM). In the two types of DM (Type I and II), Type II is the most common one all among the world.Aegle marmelos (Vilvam) is a popular medicinal plant in Siddha systems of medicine used to treat diabetes, diarrhea, Peptic ulcer, Spermatorrhoea etc. The plant Vilvam is the ‘Thalavriksham” in all Lord Shiva temples. Aegle marmelos is used for its anti-Diabetic activity all over the India by the practitioners of Indian system of medicine.

An isocratic Simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of Alprazolam and Fluoxetine HCL in tablet dosage form. Quantification was achieved by using a reversed-phase C8 column (ZORBAX C₈ Column, 5µ, 150 mm × 4.6 mm) at ambient temperature with mobile phase consisting of Mixed 50mM Phosphate buffer: Acetonitrile (30:70 pH:4.0)). The flow rate was 1.0 ml/min. Measurements were made at a wavelength of 229nm. The average retention time for Alprazolam and Fluoxetine HCl were found to be 2.15 min and 3.14. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 0.6-1.4µg/ml for Alprazolam and 48 to 112µg/ml for Fluoxetine HCl. All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of Alprazolam and Fluoxetine HCl in tablet dosage form.

An isocratic simultaneous estimation by RP-HPLC Method were developed and validated for the quantification of Hydrochlorothiazide and Irbesartan in tablet dosage form. Quantification was achieved by using a reversed-phase C18 column (Inertsil Column, 5µ, 250 mm × 4.6 mm) at ambient temperature with mobile phase consisting of Phosphate Buffer buffer(50mM): Acetonitrile (60: 40 pH: 6.0)). The flow rate was 1.0 ml/min. Measurements were made at a wavelength of 235nm. The average retention time for Hydrochlorothiazide and Irbesartan were found to be 2.85 min and 4.22. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 90-210µg/ml for Hydrochlorothiazide and 7.5 to 17.5µg/ml for Irbesartan. All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of Hydrochlorothiazide and Irbesartan in tablet dosage form.

Pellets are agglomerates of fine powders or granules of bulk drugs and excipients. They consist of small, free flowing, spherical or semi-spherical solid units, typically from about 0.5mm to 1.5mm, and are intended usually for oral administration. Pellets can be prepared by many methods, the compaction and drug-layering being the most widely used today. The main objective of this work is to develop a stable, pharmaceutically equivalent, robust and delayed release micro pellet formulation of Esomeprazole magnesium trihydrate, which is an orally administered benzimidazole anti-ulcer drug. The present investigation aimed to study two types of polymers hydroxypropyl methyl cellulose (HPMC), polyvinyle pyrolidine (PVP) to prepare the delayed release micropellets. All these formulations would be prepared in different percentages by wet granulation method. Evaluation of these micro pellets would be performed by post compression parameter, dissolution rate studies, and stability studies.

The developments of enhanced oral protein delivery technology by immediate release tablets which may release the drugs at an enhanced rate are very promising for the delivery of poorly soluble drugs high molecular weight protein and peptide. In this research work Ramipril immediate tablets were prepared by using different concentrations of the super disintegrants. Immediate release tablets are those tablets which are designed to disintegrate and release their medicaments with no special rate controlling features, such as special coating and other techniques. Ramipril is poorly soluble in water hence the basic objective of this study was to produce immediate release Ramipril tablets containing disintegrant via wet granulation.