A simple, accurate, precise and sensitive First order derivative Spectrophotometric method was developed for the estimation of Clomiphene Citrate in bulk and pharmaceutical dosage forms. The estimation of Clomiphene Citrate was carried out at maximum absorbance of 293 nm. The method was found to be linear and obeys Beer’s law in the concentration range of 10-50 mcg / ml. The developed method was validated according to ICH guidelines and was found to be accurate and precise. Thus the proposed method can be successfully applied for the estimation of Clomiphene Citrate in bulk and pharmaceutical dosage forms.

A new method has been developed for the synthesis of some N-acyl sulphonamides in the presence inexpensive and bench top reagents. The main aim of this study includes synthesis of some N-acyl sulphonamides by using acid chlorides. Various aromatic sulphonamides are reacted with acid chlorides i.e. isothalyl chloride, terythalyl chloride in the presence of base (NaHCO₃). It is a green colour reaction where the synthesis is carried out under the solvent free conditions. Totally 8 compounds of n-acyl sulphonamides were synthesized. The compounds were isolated by TLC. Structures of the synthesized compounds were confirmed by IR spectroscopy and molecular weights were confirmed by MASS spectroscopy.

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Vardenafil and Dapoxetine Hcl in combined dosage form. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Vardenafil and Dapoxetine Hcl show an isoabsorptive point at 270 nm in water. The second wavelength used is 292 nm, which is the λ-max of Dapoxetine Hcl in water. The linearity was obtained in the concentration range of 1-5 μg/ml for Vardenafil and 10-30 μg/ml for Dapoxetine Hcl. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of Dapoxetine Hcl. The method was successfully applied to pharmaceutical dosage form because no interference. The results of analysis have been validated statistically and by recovery studies. 

Aceclofenac is an NSAID (Non Steroidal Anti Inflammatory Drug) have been indicated for various painful inflammations, including post traumatic pain and proved as effective as other Non Steroidal Anti Inflammatory Drugs with lower indications of GI adverse effects and thus resulted in greater compliance with treatment. Aceclofenac fast dispersible (melt in mouth) tablets have been prepared by direct compression method. Fast disintegrating drug delivery system offers a solution for those patients having difficulty in swallowing tablets/ capsules etc. Effect of superdisintegrants such as crosscarmellose sodium and sodium starch glycolate on wetting time, disintegration time, drug content, dissolution time has been studied. Disintegration time and dissolution parameters were less in the tablets containing crosscarmellose sodium than sodium starch glycolate. All the formulation showed disintegration time in range of 85 to 130 seconds along with rapid in vitro dissolution. It was concluded that the fast dissolving tablets of the poor soluble drug can be made by direct compression technique using selective super disintegrants showing enhanced dissolution, taste masking and hence better patient compliance and effective therapy.

A new, simple, specific, accurate, precise, and rapid reverse phase high performance liquid chromatographic method was developed and validated for the determination of Tolvaptan in pure and tablet dosage forms. The HPLC separation was carried out by reverse phase chromatography on Symmetry C18 (150 x 4.6mm; 5μm) with a mobile phase consist of acetonitrile: methanol: buffer (680 mg potassium dihydrogen phosphate in      500 ml water, pH-3 adjusted with ortho phosphoric acid) in the ratio of 40:10:50 v/v delivered in isocratic mode at a flow rate of 1.5 ml/min. The Tolvaptan was quantified at 254nm. The retention time of Tolvaptan was 7.419 min. The developed method was validated according to ICH guidelines. The interday and intraday precision was found to be within limits. The developed method has adequate sensitivity and specificity for the determination of Tolvaptan in bulk and its tablet dosage forms. Accuracy (recoveries: 98.48 - 101.60 %.) and reproducibility were found to satisfactory. The developed method was found to be cost effective and was successfully employed for the determination of Tolvaptan in various pharmaceutical preparations.