A simple, Accurate, precise method was developed for the simultaneous determination and validation of the telmisartan and ramipril in Tablet dosage form. Chromatogram was run through thermo BDS (250mm 4.6mm, 5µ). mobile phase containing Buffer and Acetonitrile in the ratio of 60:40A was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.02N KH₂PO₄ buffer at P^H 3.0 temperature was maintained at 30ºC. Optimized wavelength for telmistran and ramipril was 245nm. Retention time of telmistran and ramipril were found to be 5.35min and 10.3min. % RSD of the telmistran and ramipril were found to be 1.11 and 0.518 respectively. %Recover was Obtained as 99.12 and 99.22 for telmistran and ramipril respectively. LOD, LOQ values are obtained from regression equation telmistran and rampril 9.47and1.16 and 31.57and 3.88 respectively. The HPTLC method % RSD 0.37 and 0.53 % Recover was obtained 99.85 and 99.73 respectively. The LOD LOQ was found to be 6.23 and 103 respectively.

About 95% of all new potential therapeutic drugs (APIs) exhibit low and variable oral bioavailability due to their poor aqueous solubility at physiological pH and consequent low dissolution rate. Olmesartan, a widely prescribed anti-hypertensive drug belongs to class II under BCS classification and exhibit low and variable oral bioavailability due to its poor aqueous solubility. Olmesartan tablet formulation by 2³ factorial designs for selecting the best combinations of diluents, binder and disintegrant giving fast dissolution of the drug Olmesartan.

Anisomeles malabarica (L) is a medicinal plant has been used as a folk medicine to anorexia, fevers, swelling, and rheumatism. The aim of this study deals with the investigation of antibacterial activity present in the leaves extract obtained by analytical standard polar and non-polar solvents with silver nitrate. The antibacterial properties against some pathogenic gram positive and gram negative bacteria. The minimum zone of inhibition was found in Staphylococcus aureous, E.coli, Proteus mirabilis, Staphylococcus epidermis and Klebsiella pneumoniae. The maximum zone of inhibition was found in Staphylococcus aureous, E.coli and Pseudomonas aeruginosa.

The purpose of this study was to improve the dissolution rate of a poorly soluble drug, glibenclamide using Liquisolid technique. Different LS compacts were prepared using a mathematical model to calculate the required quantities of powder and liquid ingredients to produce acceptably flowable and compressible admixture. Propylene glycol was selected as the solvent. Avicel pH 102, Aerosil 200 and cross carmellose sodium were used as carrier, coating material and disintegrant respectively for preparing the tablets. The formulations were then evaluated for their flow properties such as bulk density, tapped density, compressibility index, angle of repose and Hausner’s ratio. DSC and XRPD analysis were performed to know whether there is any interaction between drug and excipients and also to study the changes in drug crystallinity. The dissolution studies revealed that all the formulations have higher drug release rates than that of marketed and conventional tablets. The DSC and XRPD results showed that there is no interaction between the drug and excipients and also confirmed the existence of the drug in the solubilised form, which is proved by the absence of endothermic peak in DSC and reduction in the intensity of XRPD peaks. Among the different formulations prepared, LS-3 with a Lf value of 0.270 and R value of 30, was chosen as the best formulation based on its higher percentage release (91.35%). The study has produced encouraging results and it was concluded that Liquisolid technology can be used as an efficient method in improving the solubility and dissolution characteristics of poorly soluble drugs.

The aim of present investigation was to enhance the dissolution rate of a poorly water soluble drug cefpodoxime proxetil by nanosuspension formation using a solvent precipitation method. Cefpodoxime proxetil (CP), a semisynthetic β- lactum antibiotic of cephalosporin class belonging to BCS class IV with poor solubility and poor permeability is the ideal drug candidate with limited oral bioavailability when orally administered. Selected parameter of nanosuspension such as concentration of drug, solvent-anti solvent volume ratio were varied so as obtain nanoparticle within the size range less than 1μm. Characterization of the cefpodoxime proxetil of nanoparticle was carried out on the basis of scanning electron microscopy (SEM), X-ray diffraction (XRD), FTIR spectrum, particle size, and zeta potential and dissolution tester. Results signify that the combination of lowest concentration of stabilizer HPMC E50 (0.1%w/v) with low stirring speed (800 rpm) tends to achieves mallest particle size 755.6 nm and -22.6 mV, respectively. FTIR spectra shown that no chemical incompatibility between drug and polymer exist. The in-vitro drug release (%) cumulative profile of cefpodoxime proxetil nanosuspension was significantly higher (94%) as compared to marketed preparation (54%) in simulated intestinal fluid (pH 6.8).

Biological synthesis of silver nanoparticles using Calotropis giganteaflower extract was investigated and the effect of flower extract in reduction mechanism andparticle size is reported. The reduction of silver(Ag+) was monitored using UV- visible spectrophotometry and showed formation of silver nanoparticles with in 60mins. The formation of Ag-NPs was confirmed by Scanning Electron Microscopy (SEM) and are quite polydispersed, the size ranging from 10 nm to 50 nm. The formationof crystalline silver nanoparticles was confirmed using Xray diffraction analysis. Extracellular synthesis of Ag nanoparticles using flower extract appears to be cost effective, eco-friendly to that of conventional methods of nanoparticles synthesis.

The Study entitled “Assessment of Knowledge and Role of Pharmacist in Improving the Knowledge and Prevention Practices of Dengue among Selected Rural High School Children ofGuntur District” was designed to assess the awareness of dengue fever and also to evaluate the impact of an educational intervention carried out in this study. A suitable self-administered (KAP) Knowledge, attitude, awareness survey questionnaire was designed and validated by pilot study and the study was conducted among secondary school students of the government school. This prospective knowledge attitude practice(KAP) questionnaire study of 6 month duration included a total of 500 (100%) participants. An interactive educational intervention was designed for all participants of pre-KAP questionnaire survey. The impact of effectiveness of educational intervention among the secondary school students was evaluated by means of post-KAP questionnaire survey. The paired t-test and chi-square test was done by using SPSS software for statistical calculation. In our study a total of 500 participants responded and involved in the pre-KAP and post-KAP questionnaire survey. Participants involved in the study were 256 male and 244 female secondary schoolstudents of 8 th , 9th and 10 th standards. The overall response rates between pre intervention and post intervention was statistically significant (P<0.05) shows effectiveness of educational intervention for improving awareness of dengue fever among the participants. The study concluded that imparting the knowledge and awareness on dengue fever among the secondary school students by means of continuous educational intervention would bring up updated knowledge and preventive measures regardingdengue virus.

The present work reports a simple, cost effective and eco-friendly method for the synthesis of silver Nanoparticles using different ornamental flowers like Polianthes tuberosa, Tabernaemontana divaricata, Catharanthus roseus, and Chrysanthemum indicum. The extracts of each flower were prepared. The formations of silver Nanoparticles from the extracts were identified by the colour changes. The extract color changes during the formation of silver Nanoparticles from pale yellow to dark red for polianthes tuberosa, light yellow to yellowish brown for Tabernaemontana divaricata, pale yellow to reddish brown for Catharanthus roseus and colorless solution to reddish brown for Chrysanthemum indicum. Silver Nanoparticles formation was confirmed by XRD, UV, FTIR, SEM, EDX and the antimicrobial activity.

The main objective of the study is to formulate Irinotecan Hydrochloride loaded sustained release nanoparticles with the size of around 200 nm and to increase the encapsulation efficiency of the drug. The nanoparticles were prepared by simple ionic gelation method using various concentrations of chitosan and TPP. The prepared nanoparticles were evaluated for particle size, shape, charge, encapsulation efficiency, in vitro drug release and in vitro cytotoxicity. The optimised Irinotecan Hydrochloride loaded nanoparticle showed size of 172 nm with PDI of 0.36 Zeta potential of 4-±1mv, encapsulation efficiency of 85.8% and the drug release is 99 % at 24 hrs. These results demonstrate that the possibility of delivering Irinotecan Hydrochloride nanoparticles to colorectum with enhanced encapsulation efficiency.

Haemorrhoids are dilated veins within the anal canal in the sub epithelial region formed by radicals of the superior, middle, inferior rectal veins. They are divided into two types, internal and external Haemorrhoids. The internal haemorrhoids lie beneath the anal mucous membrane. Traditional Siddha system of medicine is widely practised in India. Siddha has thirty two forms of internal and external medicines. Parpam is one among the internal medicine equated to whitecalx. Velvangaparpam is a calcined form of mineral drug. My Aim of the study is to investigate the safety and efficacy of siddha drug velvangaparpam (VP).VP was evaluated for styptic activity against the standard drug Adrenochrome in Wister albino rats. They divided in to four groups, each group with 6 animals. Group 1 is the control, group 2 and 3 receive the trial drugs of dose 30 and 60mg group 4 receive the standard drug Adrenochrome (10mg/kg). Then detailed hemotological study was performed for all animals. The investigation shows a significant reduction in the Bleeding time, Clotting time, prothrombin time, and fibrinogen time. The values of the trial drug Velvangaparpam treated animals were compared with standard drug Adrenochrome 10mg/animal i.p single dose. Good Styptic activity response of trial drug’s explored.